Mass spectrometry
Mass spectrometry enables the identification and quantitation of up to hundreds of bioactive compounds in a single run. In combination with liquid chromatography (LC) and/or ion mobility separation screening, identification, and quantitative analysis of sensory active and bioactive natural products in food products, plant materials and bio fluids their metabolism, as well as the fundamental understanding of structure/activity relationships were performed.
Equipment:
| 4000 Q Trap MS/MS, AB Sciex |
| 5500 Q Trap MS/MS, AB Sciex |
| 6500 Q Trap MS/MS, AB Sciex |
| 6500+ Q Trap MS/MS, AB Sciex |
| 6600 tTOF, AB Sciex |
| Synapt G2-S HDMS, Waters |
| Xevo TQ-S, Waters |
| Vion HDMS, Waters |
Our triple quadrupole instruments are mainly used for quantitation of bioactives (targeted metabolomics), typically using the multiple reaction monitoring (MRM) mode using electrospray (ES) or atmospheric pressure chemical (APC) ionization. Additional experiments like neutral loss, precursor ion or full scan as well as information dependent acquisition (IDA) complete the analytical repertoire.
The Synapt G2-S HDMS instrument is typically used for non-targeted metabolomics approaches, offering the elemental composition of interesting metabolites. Combining the power of fast liquid chromatography (secs), high-efficiency ion mobility (IM) separation (msecs) and high resolution TOF mass spectrometry (µsecs), the three separation techniques offer complementary capacity for very complex matrices.