Separation Techniques
Efficient separation techniques are key for competitive natural product chemistry, nowadays. Available technologies range from high-throughput separation for quantitative analysis of target molecules at femto- or picogram levels all the way up to the gram-scale preparative isolation of bioactive molecules from nature’s complex compound mixtures such as, e.g. food and botanical extracts, plasma, saliva, and urine.
Equipment
Analytical separations
Electrophoresis
Ultraperformance liquid chromatography (UPLC)
High-performance liquid chromatography (HPLC)
Micro-HPLC
2-Dimensional high-performance liquid chromatography (2D-HPLC)
High-performance ion chromatography (HPIC) with pulsed amperometric detector (PAD) or conductivity detector with electrochemical suppression (sCD)
semipreparative to preparative separations
ultrafiltration (stirred cell, gas pressure filtration, tangential flow filtration)
gel permeation chromatography (GPC)
automated flash chromatography (AFC)
High-performance liquid chromatography (HPLC)
2-Dimensional high performance chromatography (2D-HPLC)
Medium pressure liquid chromatography (MPLC)
Fast centrifugal partition chromatography (FCPC)
