Substantial progress has been made in decoding the human proteome. The ProteomicsDB team led by Bernhard Kuster (Technische Universität München, TUM) presents its findings back to back with a similar study by Akilesh Pandey’s group (Johns Hopkins University) in the latest edition of Nature.
The TUM…
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Low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system…
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How does an ordinary somatic cell become cancerous? What are the distinct characteristics of tumor cells that make them divide uncontrollably? Previously, researchers were primarily interested in mutations in DNA, which is the blueprint for proteins. But since proteins are ultimately responsible for…
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We present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different search…
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Protein sequence databases are indispensable tools for life science research including mass spectrometry (MS)-based proteomics. In current database construction processes, sequence similarity clustering is used to reduce redundancies in the source data. Albeit powerful, it ignores the…
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