16 Apr 2013 | Technical Advance DOI: 10.3410/f.717997979.793474359

O-GlcNAc transferase (OGT) glycosylates a large and growing list of human proteins. Here, the authors identify a surprisingly large number putative target proteins using azo-GlcNAc (GlcNAz), on-resin Click chemistry, and selective proteolysis. Notably, they validate a small subset of these by identifying specific sites of O-GlcNAcylation. While more work is needed to validate low-abundance targets, this study provides an unbiased, top-down analysis of the effects of OGT regulation in human cells.

Suzanne Walker and Lincoln Pasquina.
F1000 Chemical Biology > Biocatalysis
Harvard Medical School, Boston, MA, USA.

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